Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Karimi-Busheri, F.; Weinfeld, M.
    Purification and substrate specificity of polydeoxyribonucleotide kinases isolated from calf thymus and rat liver (1997), J. Cell. Biochem., 64, 258-272.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.78 nucleus
-
 Rattus norvegicus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 Mg2+
-
 Rattus norvegicus
2.7.1.78 Mg2+
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.78 60000
-
 x * 60000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Rattus norvegicus
-
 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA Bos taurus involved in repair of DNA single strand breaks ADP + 5'-phospho-DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Bos taurus
-
 calf
-
2.7.1.78 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 10860fold, to near homogeneity Rattus norvegicus
2.7.1.78 6717fold, to near homogeneity Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.78 liver
-
 Rattus norvegicus
-
2.7.1.78 liver
-
 Bos taurus
-
2.7.1.78 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.78 0.0012
-
 purified enzyme Bos taurus
2.7.1.78 0.0028
-
 purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Rattus norvegicus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA minimal length of the substrate is 7-8 nucleotides, optimal size is more than 18 nucleotides in length Rattus norvegicus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA minimal length of the substrate is 7-8 nucleotides, optimal size is more than 18 nucleotides in length Bos taurus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA no preference for overhanging 5'-ends Bos taurus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA single stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA single stranded DNA Bos taurus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA specific for DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA specific for DNA Bos taurus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA micrococcal-nuclease-treated calf thymus DNA Bos taurus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA involved in repair of DNA single strand breaks Bos taurus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + synthetic oligonucleotide
-
 Rattus norvegicus ADP + oligonucleotide 5'-phosphate
-
 ?
2.7.1.78 ATP + synthetic oligonucleotide low activity with homopolymers such as oligo(dA)24 and oligo(dT)24 Bos taurus ADP + oligonucleotide 5'-phosphate
-
 ?
2.7.1.78 additional information substrate specificity Bos taurus ?
-
 ?
2.7.1.78 additional information enzyme also has DNA 3'-phosphatase activity Rattus norvegicus ?
-
 ?
2.7.1.78 additional information enzyme also has DNA 3'-phosphatase activity Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.78 ? x * 60000, SDS-PAGE Rattus norvegicus
2.7.1.78 ? x * 61000-62000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.78 More enzyme also possesses a 3'-phosphatase activity Rattus norvegicus
2.7.1.78 More enzyme also possesses a 3'-phosphatase activity Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.78 37
-
 assay at Rattus norvegicus
2.7.1.78 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 5.5
-
 assay at Rattus norvegicus
2.7.1.78 5.5
-
 assay at Bos taurus
2.7.1.78 6
-
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP dependent on Rattus norvegicus
2.7.1.78 ATP dependent on Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.78 Bos taurus
-
-
 8.5